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A similar approach has been used to identify genes that are differentially expressed in human prostate cancer. Yang et al combined suppression subtractive hybridisation SSH with microarray technology to identify differentially expressed vidalista 40 reviews genes in ER-positive MCF-7 and T47D and ER-negative MDA-MB 23 and HBL100 breast cancer cell lines. SSH is based on the technique of representational difference analysis RDA reviews maxviril but modified to analyse mRNA abundance.

SSH was first performed using ER-positive MCF-7 mRNA as a tester and ER-negative MDA-MB-231 cDNA as a driver. I CAN'T HEAR YOU OVER THE SOUND? OF RUNNING UBUNTU filagra 50 mg datalife engine demo. A set of 332 cDNA clones which should be enriched for kamagra viagra reviews genes overexpressed in MCF-7 cells compared to MDA-MB-231 cells was identified and arrayed. When microarrays were hybridised to probes prepared from ER-positive and ER-negative breast cell lines, 10 clones were found to be overexpressed at least fivefold in ER-positive cells.