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Although selection of replicons resistant to 2-C-methyl-nucleosides has shown that HCV is rapidly able to discriminate between antiviral agents and natural nucleosides81, in vitro studies have shown that metapro 50mg tab 10`s replicons carrying these mutations showed decreased replication fitness83, 87, 88, 89. Or the Starbucks.? order tadarise-40. There are several binding sites for non-nucleoside analogues within the NS5B polymerase Fig. Several mutations have been identified as determinants for resistance to non-nucleosides. For example, it has been demonstrated in vitro that replacement of P495 with alanine or leucine strongly reduces affinity for non-nucleoside inhibitors83, 89. Such a mutation decreases the efficiency of viral replication, but viral fitness can be restored by mutations elsewhere in the NS5B coding region83.

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